Author Archives: James Cawse

HT Experimental Strategy in Education

I recently spent two valuable days at the Freeslate Forum in San Francisco. Two of the case studies were on the use of high throughput tools in more academic settings, by Emory Chan of The Molecular Foundry at Lawrence Berkeley National Laboratory and by Scott Virgil at the Center for Catalysis and Chemical Synthesis (3CS)… Read More »

HT Experimental Strategy 4. Resources & Factors

Landscapes with moderate degrees of ruggedness share a striking feature: it is the highest peaks that can be scaled from the greatest number of initial positions! (Stuart Kauffman, At Home in the Universe)  In the previous step, we quickly found that even with a relatively modest selection of factors and levels, the combinatoric explosion can… Read More »

HT Experimental Strategy. Step 3. Experimental Space

“To boldly go where no one has gone before…” (Star Trek)  If a project is worth the investment of a high throughput laboratory, it must have an important element of discovery, pushing away from the familiar into different spaces. But the simple facts of chemistry generate enormous numbers of combinations that quickly become a nightmare… Read More »

High Throughput Strategy. Step 2: HT Measurements

Step 2. What are we measuring? Are we measuring it well? Not everything that can be counted counts and not everything that counts can be counted. (Albert Einstein)  In the previous step, we discussed ways to move from the goals and objectives of the business to unbiased, specific and measurable laboratory objectives. Now we need… Read More »

Experimental Strategy: A Blueprint. Designs

Step 5. What do we do in the laboratory? “You can’t fix by analysis what you bungled by design.” – By Design (Light, Singer and Willett) This is the part of the experimental strategy process where most Design of Experiments (DOE) classes start. The student is taught about Analysis of Variance (ANOVA), factorial designs, and… Read More »

Experimental Strategy: a Blueprint. Resources

Step 4. Do we have the resources?  “The amateurs discuss tactics: the professionals discuss logistics.” – Napoleon Bonaparte  It is really frustrating to get half way through an experiment and then get pulled off for “higher priority” work. If that happens during a designed experiment, the return for your effort could be ZERO! In this… Read More »